Abstract: In order to solve the problems of residual polyacrylamide( PAM) in the coal slime water,such as reducing the flotation performance and polluting the environment. Firstly,the mixed strain was formed by combining Phanerochaete chrysosporium( P. chrysosporium) and Rhodopseudomonas spheroides( R. spheroides) in equal volume. The effects of environmental factors and nutritional conditions on PAM degradation were investigated by mixed strain. Secondly,the degradation products were analyzed by FT-IR and viscosity method. Finally,the enzyme activity of degradation culture was tested. The results indicate that P. chrysosporium and R. spheroides can degrade PAM with degradation rate of 30.2% and 24.9%,respectively when added separately. The mixed strain degradation rate of PAM reaches 34.3%. The degradation rate of PAM reaches 37.5% under the optimal conditions. Different conditions will lead to significantly different degradation effects. A small amount of the glucose and the ammonium tartrate can promote the degradation,and the temperature has a significant effect on the degradation of PAM. The amide group of the side chain of PAM is converted into carboxyl group,which can be used as nitrogen source for microorganisms. The carbon chain skeleton of PAM is broken under the action of strong oxidase,resulting in the decline of molecular weight,which can provide carbon source for microbes. Laccase,manganese peroxidase and lignin peroxidase are detected in the degraded fermantation liquid with enzyme activity of 16.48 U/m L,12.09 U/m L and 6.73 U/m L,respectively.