Abstract: A multiphase flow model was used to simulate the liquid-solid flow in the interference bed separator, and experimental studies were carried out based on the results of the simulation to obtain the separation efficiency and optimal operating parameters of the dual-chamber interference bed. The simulation study showed that for the 1−0.5 mm particle size, under the working condition of a circulation flow rate of 8 L/min, the separation efficiency of particles with a density of 1.25 kg/L was 78.7%; for the 0.5−0.25 mm particle size, under the working condition of a circulation flow rate of 3 L/min, the separation efficiency of particles with a density of 1.25 kg/L was 84.3%. The experimental results indicated that under the feeding particle size of 1−0.5 mm and water flow velocity of 8 L/min, the possible deviation E value could reach the minimum value of 0.048, which is less than 0.1; under the same conditions, the Ep value of the dual-chamber interference bed separator is lower than that of the single-chamber interference bed separator. Judging from the possible deviation values, the separation effect of the dual-chamber interference bed is better than that of the single-chamber interference bed.