Abstract: The residue of nitrate in industrial wastewater has a great negative impact on the ecological environment. Nitrogen is green, non-toxic and does not need secondary treatment, so it is considered to be an ideal target product for nitrate removal of wastewater. Electrocatalytic reduction is a sustainable method for nitrate removal from wastewater, which is still faced with some problems, such as slow kinetics, low selectivity of target products and poor stability. Cu metal is one of the commonly used electrocatalysts for nitrate reduction, but in the process of electrode polarization, Cu atoms are easy to migrate and agglomerate, resulting in the decline of reaction activity, and its durability is often poor in industrial applications. Therefore, higher requirements are put forward for the electrochemical performance and stability of the catalyst. In this paper, mesoporous carbon in-situ limited Cu nanoparticles CNTs@mesoC@Cu catalysts were synthesized by molecular-mediated interfacial assembly strategy, and their electrocatalytic performance and reaction mechanism for selective reduction of nitrate were studied. Under the synergistic action of 3% concentration of Cl-, after potentiostatic reduction for 24 hours, the CNTs@MesoC@Cu catalyst can reduce the total nitrogen concentration C (NO3--N) of the simulated wastewater from 140ppm to 8.5ppm, and the nitrogen selectivity is higher than 95%, which meets the comprehensive sewage discharge standard. In addition, after 120 h stability test, the selectivity of the catalyst for nitrogen is still above 96%, which proves that it has considerable stability. The catalyst shows good performance in the selective reduction of nitrate. The binding of conductive carbon nanotubes @ mesoporous carbon substrate limits the copper active center on the surface, which enables CNTs@MesoC@Cu to maintain stable electrochemical nitrate catalytic activity. This study provides a feasible solution for the large-scale industrial application of copper-based electrocatalysts.