洁净煤技术

2020, v.26;No.130(06) 138-143

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

放线菌筛选及降解光氧化褐煤工艺条件研究
Study on actinomycetes screening and technological conditions for biodegradation of photooxidized lignites

李建涛;刘向荣;石晨;庄肃凯;
LI Jiantao;LIU Xiangrong;SHI Chen;ZHUANG Sukai;College of Chemical Engineering and Modern Materials,Shangluo University;Shaanxi Key Laboratory of Comprehensive Utilization of Tailings Resources;College of Chemistry and Chemical Engineering,Xi'an University of Science and Technology;

摘要(Abstract):

煤的微生物转化是低阶煤高效清洁利用的有效途径,放线菌是降解低阶煤的主要作用菌之一。光氧化预处理低阶煤能够增加低阶煤的含氧量,有利于提高微生物对煤的降解率。为了获得降解褐煤的高效放线菌菌株,对5种放线菌和4种光氧化褐煤进行了菌-煤匹配试验,经过对比5种放线菌对4种光氧化褐煤的降解效果,最终筛选出的优势放线菌降解菌株为绿孢链霉菌。以绿孢链霉菌降解光氧化褐煤所得降解液在450 nm处的吸光度值为指标,通过单因素试验探讨了绿孢链霉菌降解光氧化内蒙胜利褐煤的工艺条件,确定的最佳工艺条件为:20 mL培养基的加煤量和接种量分别为0.2 g和3.0 mL,培养时间为10 d,培养箱振荡频率为160 r/min,煤样粒度为0.150~0.075 mm,培养温度为28℃。依据最佳工艺条件,进一步研究了绿孢链霉菌对光氧化云南昭通褐煤、光氧化山西浑源褐煤和光氧化内蒙元宝山褐煤的降解效果,结果表明:绿孢链霉菌对3种光氧化褐煤的降解效果均有一定程度的提升。因此,以内蒙古胜利褐煤为样本确定的绿孢链霉菌降解光氧化褐煤的工艺条件对不同光氧化褐煤的降解过程具有一定的普适性,可作为研究绿孢链霉菌降解光氧化褐煤的较优工艺条件参考使用。
Microbial degradation of coal is an effective way for efficient and clean utilization of low rank coal,and actinomycetes are one of the main microbes to degrade low rank coal.Photooxidation pretreatment of low rank coal can increase the oxygen content of low rank coal,which will help to improve the biodegradation rate of coal by microorganism.In order to obtain high-efficiency biodegradation strain of lignite,five actinomycetes and four photooxidized lignites were tested by actinomycete-coal matching experiments.After comparing the degradation effect of 5 kinds of actinomycetes and 4 kinds of photooxidation lignite,the dominant strain selected was Streptomyces viridosporous.Taking the absorbance value at 450 nm of the degradation solution obtained by Streptomyces viridosporous as the index,the single factor experiments were carried out to determine the technological condition of biodegradation of Inner Mongolia Shengli lignite by Streptomyces viridosporous. Finally,the determined optimum technological conditions were listed as follows: coal slurry mass concentration of 0.2 g/20 mL,inoculums size of 3.0 mL/20 mL,incubation time of 10 d,oscillation frequency of 160 r/min,coal particle size of 0.150-0.075 mm and incubation temperature of 28 ℃ .According to this condition,the degradation effects of Streptomyces viridosporous degrading GZTH,GHYH and GYBH were studied.The results show that under this condition,the degradation effects of Streptomyces viridosporous degrading the three lignites are all improved to some extent. It can be seen that the process conditions of photooxidation lignite degraded by Streptomyces viridosporous taking Shengli lignite in Inner Mongolia as the sample has a certain universality for the degradation process of different photooxidation lignite,which can be used as a reference for the study of the better process conditions for the degradation of photooxidation lignite by Streptomyces viridosporous.

关键词(KeyWords): 放线菌;褐煤;微生物降解;光氧化
actinomycete;lignite;biodegradation;photooxidation

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(21373158);; 陕西科技厅中澳合作项目(2013KW17)

作者(Author): 李建涛;刘向荣;石晨;庄肃凯;
LI Jiantao;LIU Xiangrong;SHI Chen;ZHUANG Sukai;College of Chemical Engineering and Modern Materials,Shangluo University;Shaanxi Key Laboratory of Comprehensive Utilization of Tailings Resources;College of Chemistry and Chemical Engineering,Xi'an University of Science and Technology;

Email:

DOI: 10.13226/j.issn.1006-6772.19110607

参考文献(References):

文章评论(Comment):

序号(No.) 时间(Time) 反馈人(User) 邮箱(Email) 标题(Title) 内容(Content)
反馈人(User) 邮箱地址(Email)
反馈标题(Title)
反馈内容(Content)
扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享